Plasmid

Part:BBa_K1074001:Design

Designed by: Changlong Zhao   Group: iGEM13_USTC_CHINA   (2013-09-09)


PHT43


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1255
    Illegal EcoRI site found at 3507
    Illegal XbaI site found at 2911
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1255
    Illegal EcoRI site found at 3507
    Illegal NheI site found at 1091
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1255
    Illegal EcoRI site found at 3507
    Illegal BglII site found at 2978
    Illegal BglII site found at 6059
    Illegal BamHI site found at 2905
    Illegal XhoI site found at 6063
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1255
    Illegal EcoRI site found at 3507
    Illegal XbaI site found at 2911
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1255
    Illegal EcoRI site found at 3507
    Illegal XbaI site found at 2911
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6954
    Illegal SapI.rc site found at 4131
    Illegal SapI.rc site found at 8036


Design Notes

Not conform to the BioBrick standard


Source

Bought from MoBiTec company

References